Characterization of deoxyribonucleic acid from Yersinia pestis by ethidium bromide-cesium chloride density gradient centrifugation.
نویسندگان
چکیده
No plasmids were detected in cells of Yersinia pestis which were bacteriocinogenic and positive for three additional determinants of virulence known to be lost at high frequency.
منابع مشابه
Method for obtaining more-accurate covalently closed circular plasmid-to-chromosome ratios from bacterial lysates by dye-buoyant density centrifugation.
A method that gives high recovery of deoxyribonucleic acid (DNA) from crude bacterial lysates using ethidium bromide-cesium chloride density gradient centrifugation is presented. After Pronase digestion and shearing of the lysate, essentially 100% recovery of chromosomal DNA and a reproducible recovery of covalently closed circular (CCC) plasmid DNA is obtained for a specific plasmid in a given...
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ورودعنوان ژورنال:
- Infection and immunity
دوره 5 4 شماره
صفحات -
تاریخ انتشار 1972